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The purposes of this study are the identification and determination of metabolites in the isolated rat liver perfusate of carbon disulfide by two-dimentional thin-layer chromatography and high performance liquid chromatography for understanding the metabolism of carbon disulfide. 2-Thio-thiazolidine-4-carboxylic acid(TTCA) was synthesized by the reaction of carbon disulfide and cysteine, and confirmed by two-dimentional thin-layer chromatography, high performance liquid chromatography, UV spectroscopy, and IR spectroscopy. The absorbance of UV detector for the simultaneous determination of TTCA and thiocarbamide was 254 ㎚ although their maximum spectra were 273 ㎚ and 237 ㎚, respectively. Two kinds of the developing solvent in the two-dimentional thin-layer chromatography were 2-butanol : 80% HCOOH : H₂O(7 : 2 : 1) as the first developing solvent and 2-propanol : H₂O (4 : 1) as the second developing solvent. After perfusion of carbon disulfide (8274.23 umol), the amount of TTCA and thiocabamide of the perfusate(100 ㎕) were 12.02 - 16.43 umol and 5.25 - 8.l5㎍, respectively. The mean amount of them were 14.08 umol and 6.41 umo1 respectively, and the former was 2.20 times greater than the latter. For conforming the mechanism of formations of TTCA and thiocarbamide in vivo, we have to clarify whether the reactions between carbon disulfide and ammonia, ammonium salts, amides, cysteine, cystine, or proteins will be formed in vitro.
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